White Processed Versatile Plastic
Slice incubation chamber
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$25.23
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Product Description
Slice incubation chamber insert for electrophysiology.
A handy insert that can be used in combination with some 150/200mL beakers to incubate slices for electrophysiology. To use, glue netting to the bottom of the holding part (either specialized slice netting or from netted cloth, e.g. pantyhose) and trim the flanges for a snug fit in your beaker.
Sizing your beaker
The "small" and "medium" insets fit in beakers with an inner diameter between 54mm and 64mm. The "Large" inset should fit in beakers of 64mm to 68mm. The outer flanges can be easily trimmed with strong scissors or shears to create a snug fit.
The "small" and "medium" insets are identical in outer dimensions, but differ in the number of divisions: the "medium" inset is divided into 6 chamers, while the "small" inset is divided into 8 chambers.
Oxygination / liquid flow
The central canal has a strut at the bottom to ensure your oxygen tube will not drop down too far, and intake holes to facilitate liquid flow. When oxygen is flowing, it will push up the liquid inside the canal, forcing a steady and even flow of oxyginated ACSF. Slice holding area is divided in 6 or 8 compartments, which are big enough to hold a sagital or coronal mouse brain slice. Larger rat brains may not fit as well, though.
Performance so far
We currently use two "medium" sized inserts in our lab, to relative success. This replaced home-made solutions based on glueing bits of falcon tube together, and was a definite improvement. Note that some discoloration can occur, but after a year of use we have not seen drastic side-effects yet.
A larger-sized version for 400mL beakers is available, but as of yet untested. The default "white strong and flexible" material worked well for us, though other plastics could be tried. Metals are not recommended in combination with glass / perspex beakers, for obvious reasons, though could be tried if strong cleaning agents are preferred.
STL files
To print these yourself, see the STL files on Thingiverse: https://www.thingiverse.com/thing:916305
A handy insert that can be used in combination with some 150/200mL beakers to incubate slices for electrophysiology. To use, glue netting to the bottom of the holding part (either specialized slice netting or from netted cloth, e.g. pantyhose) and trim the flanges for a snug fit in your beaker.
Sizing your beaker
The "small" and "medium" insets fit in beakers with an inner diameter between 54mm and 64mm. The "Large" inset should fit in beakers of 64mm to 68mm. The outer flanges can be easily trimmed with strong scissors or shears to create a snug fit.
The "small" and "medium" insets are identical in outer dimensions, but differ in the number of divisions: the "medium" inset is divided into 6 chamers, while the "small" inset is divided into 8 chambers.
Oxygination / liquid flow
The central canal has a strut at the bottom to ensure your oxygen tube will not drop down too far, and intake holes to facilitate liquid flow. When oxygen is flowing, it will push up the liquid inside the canal, forcing a steady and even flow of oxyginated ACSF. Slice holding area is divided in 6 or 8 compartments, which are big enough to hold a sagital or coronal mouse brain slice. Larger rat brains may not fit as well, though.
Performance so far
We currently use two "medium" sized inserts in our lab, to relative success. This replaced home-made solutions based on glueing bits of falcon tube together, and was a definite improvement. Note that some discoloration can occur, but after a year of use we have not seen drastic side-effects yet.
A larger-sized version for 400mL beakers is available, but as of yet untested. The default "white strong and flexible" material worked well for us, though other plastics could be tried. Metals are not recommended in combination with glass / perspex beakers, for obvious reasons, though could be tried if strong cleaning agents are preferred.
STL files
To print these yourself, see the STL files on Thingiverse: https://www.thingiverse.com/thing:916305
Details
What's in the box:
Chamber Small 8
Dimensions:
Success Rate:
First To try.
What's this?
Rating:
Mature audiences only.
