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Bufavirus was identified in 2012 in viral metagenomic analysis of fecal samples from diarrheic children in Burkina Faso. The obtained sequence reads from one sample showed significant similarities to parvovirus sequences, and after filling the gaps between the NGS reads by PCR, a nearly full-length sequence of 4921 bp was constructed showing <39% and <31% identity of the NS1 (non-structural protein 1) and VP1 protein (viral capsid protein 1), respectively, to previously known protoparvoviruses. A unique middle open reading frame (ORF) of 130 amino acids was also identified that did not show similarity with any other parvovirus sequences. Further PCR analysis of 98 rotavirus antigen-negative diarrheal fecal samples from Burkina Faso showed the presence of BuV DNA in 4/98 (4.1%) samples.