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CRISPR-Cas9 5AXW 3d printed

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Glossy Full Color Sandstone
CRISPR-Cas9 5AXW 3d printed
CRISPR-Cas9 5AXW 3d printed

DIGITAL PREVIEW
Not a Photo

CRISPR-Cas9 5AXW

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$33.65
3D printed in fully colored material with a glossy and smooth finish.
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Product Description
The RNA-guided DNA endonuclease Cas9 cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to the guide RNA. Recently, we harnessed Staphylococcus aureus Cas9 (SaCas9), which is significantly smaller than Streptococcus pyogenes Cas9 (SpCas9), to facilitate efficient in vivo genome editing. Here, we report the crystal structures of SaCas9 in complex with a single guide RNA (sgRNA) and its double-stranded DNA targets, containing the 5'-TTGAAT-3' PAM and the 5'-TTGGGT-3' PAM, at 2.6 and 2.7 Å resolutions, respectively. The structures revealed the mechanism of the relaxed recognition of the 5'-NNGRRT-3' PAM by SaCas9. A structural comparison of SaCas9 with SpCas9 highlighted both structural conservation and divergence, explaining their distinct PAM specificities and orthologous sgRNA recognition. Finally, we applied the structural information about this minimal Cas9 to rationally design compact transcriptional activators and inducible nucleases, to further expand the CRISPR-Cas9 genome editing toolbox. 
Details
What's in the box:
exsml_CRISPR_Cas9_ml_vC6_x40
Dimensions:
3.8 x 4.83 x 3.05 cm
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1.5 x 1.9 x 1.2 inches
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Success Rate:
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Rating:
Mature audiences only.
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